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NPDN National Meeting 2007

Poster Abstract

3

Diagnostic and Detection Efforts: HLB (Citrus Greening) and the NPDN

Authors: CARRIE L. HARMON (1), Manjunath L. Keremane (2), Tim S. Schubert (3), Anne Vitoreli (4)

Affiliations: (1) Southern Plant Diagnostic Network, Department of Plant Pathology, UF, Gainesville, FL, USA; (2) USDA ARS Citrus Germplasm Repository, Riverside, CA, USA; (3) Division of Plant Industry, Florida Dept.of Ag and Consumer Services., Gainesville, FL, USA; (4) Plant Disease Clinic, University of Florida, Gainesville, FL, USA

Huanglongbing (HLB) is caused by a phloem-limited, psyllid-transmitted fastidious bacterium, Candidatus Liberibacter asiaticus. The pathogen is on the USDA List of Select Agents, which indicates its bioterrorism potential and importance to citrus production, and complicates development of improved diagnostic methods. Current approved methods require specific real-time PCR technology and are complicated by the difficulty with which DNA is extracted from citrus tissue, and the low titer and irregular distribution of the pathogen in the host. In addition, there are indications that symptoms develop late in the process of host colonization; current diagnostic tests and resulting management efforts may be too late to effectively curtail pathogen spread by the psyllid vector. During initial phases of HLB geographical delimitation, NPDN diagnosticians have contributed to surge sample diagnosis, but limitations in national lab capacity remain, so management decisions are based on representative plants, not all plants. Since HLB was first detected in Florida in Fall 2005, tremendous national and regional efforts have been expended to train diagnosticians, develop new methods, and educate first detectors. The NPDN and its partners have contributed to a wealth of information and resources that have supported the national effort to protect US citrus production.

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